Differentially expressed genes in Penaeus monodon hemocytes following infection with yellow head virus.

نویسندگان

  • Siriporn Pongsomboon
  • Sureerat Tang
  • Suleeporn Boonda
  • Takashi Aoki
  • Ikuo Hirono
  • Motoshige Yasuike
  • Anchalee Tassanakajon
چکیده

A cDNA microarray composed of 2,028 different ESTs from two shrimp species, Penaeus monodon and Masupenaeus japonicus, was employed to identify yellow head virus (YHV)-responsive genes in hemocytes of P. monodon. A total of 105 differentially expressed genes were identified and grouped into five different clusters according to their expression patterns. One of these clusters, which comprised five genes including cathepsin L-like cysteine peptidase, hypothetical proteins and unknown genes, was of particular interest because the transcripts increased rapidly (< or = 0.25 hours) and reached high expression levels in response to YHV injection. Microarray data were validated by realtime RT-PCR analyses of selected differentially expressed transcripts. In addition, comparative analysis of the hemocyte transcription levels of three of these genes between surviving and non-surviving shrimp revealed significantly higher expression levels in surviving shrimp.

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عنوان ژورنال:
  • BMB reports

دوره 41 9  شماره 

صفحات  -

تاریخ انتشار 2008